The John Deere 320 skid steer loader has a John Deere 4042 turbocharged, four-cylinder, diesel engine producing 62 horsepower (HP). It has an operating weight of 6,435 pounds (2,918.9 kg). It comes standard with a vertical-lift boom with cushioned boom cylinders, 60/40 weight distribution, low center of gravity, long wheelbase, high ground clearance, Quick-Tach hitch, serpentine belt, hydraulic valve lifters, and wet-disc park brakes.

The 320 has a hydraulic pump that delivers fluid at a rate of 19 gallons (72 L) per minute at a system pressure of 3,450 pounds per square inch (PSI) (23,770 kPa).